Toxicological and microbiological quality of water from artisian wells in the city of Realeza - Paraná

Abstract

In municipalities in the Southwest of Paraná, including Realeza, a considerable portion of the population consumes water from artesian wells with quality monitored through traditional parameters, that is, physical-chemical and microbiological parameters, but with unknown toxicological parameters. The present work, aiming to contribute with toxicological evaluations, aimed to analyze the cytogenotoxicity of water from rural and urban artesian wells in the city of Realeza - PR, through the use of the Allium cepa system. To carry out the analysis, water was collected from artesian wells, chosen at random, and following the registration list present at the City Hall/Sanitation. Allium cepa bulbs were exposed to water samples collected in triplicate, with negative control distilled water and positive control aqueous solution of copper sulphate 0.0006 for 72 hours. Subsequently, the roots were submitted to the Feulgen protocol with modifications, for the preparation of slides and verification of alterations in the values ​​of Mitotic Index and Chromosomal Alterations. From the analysis, alterations in the values ​​of Mitotic Index and Chromosomal Alterations were observed. The results obtained suggest that the water from artesian wells has the potential to damage the genome of eukaryotic organisms.